Melanotan 1 Lab Studies And Research/Conclusions
Melanotan I or afamelanotide is a synthetic hormone, which is a variation of the naturally produced alpha-melanocyte-stimulating hormone or alpha-MSH. Alpha-melanocyte hormone is produced by the body and it is essential in stimulating natural tanning process. Tanning is essential for the skin because it prevents over-exposure to the harmful ultraviolet rays of the sun. The alpha-melanocyte stimulating hormone activity is through the activation of specific melanocortin receptors, the receptors are important in the production of melanin from eumelanin. Melanogenesis is an essential process because without it the cells would be affected by the rays. It is important not to confuse melanotan with melatonin, which is a peptide, which regulates circadian rhythms. Circadian rhythm is a natural process or biological clock, which controls sleep and other activities.
Mechanism of Action
The mode of action of melanotan 1 is by mimicking the natural skin pigmentation process and the original discovery of the hormone is credited to researchers from Arizona University. The scientists in the university were looking for an alternative therapy to deal with skin cancer and related conditions. It was through the study that they identified a better alpha-melanocyte-stimulating hormone that they called melanotan later melanotan I. Studies have shown that the synthetic peptide is a thousand times more effective than the natural hormone because it has a better half life . In addition, the signaling of various molecules in the biological system is important in the activation and the conversion of eumelanin to melanin in the skin or sunless tanning . The molecule selectively binds to the melanocortin receptors or MC1R receptors on the epidermal layers.
Research shows that when the peptide is injected on test organisms it leads to tanning or formation of a brown pigmentation on the skin. The various signaling cascades that are accentuated by the peptide are known to bring out a positive feedback mechanism and eventually help in self tanning. Eumelanin is a brown or black photoprotective pigment, which is essential in preventing the epidermal cells from over-activation by the ultraviolet rays. Research shows that melanotan 1 act by activating adenylate cyclase when adenylate cyclase is activated it causes the production of cyclic GMP. The cyclic GMP, on the other hand, is essential for activating protein kinase An or PKA. The activation of PKA is important in inducing the phosphate group of the adenosine molecule and this adds a phosphate molecule to the respond binding element (CREB). The phosphorylation of CREB results in the binding of the cyclic AMP response element in the microphthalmia transcription factor gene or MITF. The binding aids in the synthesis of MIFT proteins and the activation of receptors such as MC1R.
Research on Mice
Melanotan I has been under investigation for its potency to induce sexual arousal, this is according to research done on mice. When the hormone is injected, it induces the males to seek the female mice, while the female mice exhibited lordosis, solicitation, and reddening of sexual organs. It is imperative to note that all these is for research purposes and the peptide promotes the production of melanin from the epidermal layers of the skin. The analogs of the natural alpha-melanocyte hormone are under study for melanin production. Ultraviolet rays can be quite dangerous to the epidermal cells and some of the effects include dry growths, lesions, patches, sores, lumps, burning sensations among other conditions.
One feature of the condition is that the victims of these conditions often produce the peptide but the receptors have failed and do not respond to the peptide or the receptor ligand have failed. Increasing the levels of the alpha-melanocyte-stimulating hormone does not affect anything in this context because it is receptor failure. It is imperative to understand that the rapid stimulation of the receptors can cause the activation of Melanotan 1. It is essential to know that the process usually requires UV light, although there can be some minor variations in the tanning of the body, the half-life of the peptide is enhanced therefore it will reach all the required areas and exert optimal functionality.
The research from the study shows that there are some expected side effects when the peptide is administered, some of the reactions experience include site reactions, fatigue among others. However, there are no significant changes in the hematological and blood chemistry parameters. It is essential that when you are conducting any kind of research, always strive to get ideal conditions that you can have all the situations at hand. When the conditions are more or less constant, there is a minimal chance of getting errors in the study or result.
From day one to several weeks until the desired self-tanning is achieved. The desired dosage administered to the test organism is 1 mg per day and the dosage may be increased to 2-3mg per day depending on the level of activation and the production of the melanin in the skin. It is important to understand that once you have reached a certain limit with the test subject there can be no further change because the optimal production of the body has been reached. Just like any other peptide in the market, the Melanotan 1 is supplied in freeze-dried vials. The multi-use vial is susceptible to changes in pressure and temperature, therefore, it should be handled with care when transporting and when storing. There is misleading information circling around that the peptide may stay active for weeks and they are not affected by the parameters. When you buy melanotan upon reception of the peptide, it should be kept in a vial and fridge where it will stay active. It is recommended that you pick only the amount of the peptide required for one study and not the entire peptide.
The preparation of the peptide begins with the picking of the vial from the vendor and storing it well, once you have you hypothesis and research methodology then you can remove the only required amount for the study and in this context, 10 milligrams, and you can reconstitute it with 5 ml of bacteriostatic water or saline solution. The concentration of the peptide is usually dependent on the volume used in the process. If you are using 10mg of the peptide then mix with 1 ml of water and the solution will have a concentration of 10mg/1ml or in non-technical language 10 milligrams of the peptide in 1 ml of bacteriostatic water. It is imperative to note that a further dilution will affect the ability of the peptide to induce the desired result.